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Thermal Gradient Ring (TGR)

Product Code:35550

The TGR can clearly discriminate temperature-dependent phenotypes or temperature dependent drug effects.

It records and analyses thermal preference phenotyping in mice. For neuropathic pain studies, peripheral neuropathy, temperature sensitivity and insensitivity assessment in basic research, phenotyping and drug screening.

The animal is freely moving, its position is tracked by a camera, and no user intervention is required to gather time spent in each temperature zone (24 zones in duplicate) and many other parameters.

In the TGR mice can freely move around the ring floor, thereby avoiding the stereotypical habit that mice have of staying in a corner, as occurs in rectangular systems.

It is a cost effective and bias-free tool to obtain reproducible data in a very broad range of thermal stimulation experiments.

This product warranty can be extended up to 24 Additional Months.
Discover more HERE!


  • The cellular and molecular mechanisms of temperature sensing receptors (e.g. transient receptor, TRP, channels) and thermoregulation are the subject of intensive studies in neuropathic pain, peripheral neuropathy and temperature preference in general.
  • The novel circular thermal gradient assay opens new opportunities for thermal preference and avoidance and addresses limitations imposed by classic linear equipment (lack of animal freedom, limited thermal choice, inability to make discerning exploratory behaviour, experimental biases).
  • The TGR is based on the paper "Comprehensive thermal Preference Phenotyping in Mice using a Novel Automated Circular Gradient Assay”
  • The TGR can clearly discriminate temperature-dependent phenotypes or drug effects

Novel device, bias-free data, cost effective

  • The Thermal Gradient Ring has a 45 cm inner diameter, 57 cm outer diameter, and 24 cm height.
  • A camera is located on the upper side of the apparatus, which includes an infrared camera and an infrared transmissive inner wall.
  • In the TGR mice can freely move around the ring floor, thereby avoiding the stereotypical habit that mice have of staying in a corner, as occurs in rectangular systems.
  • The behavioral data is obtained by video-tracking and can be videotaped to then let the software automatically analyse the “spent time”, “travel distance” or “speed”.
  • A “preference temperature” can be defined as the mean value using the zone temperature and “spent time” and the % of time spent above this temperature will be automatically calculated.



New circular design, internal diameter 45cm, outer diameter 57cm

Duplicate values, no border effects, no spatial cues

Thermal Insulated Ring-shaped Aluminum Runway

More sensitive than previous methods: bias-free, reproducible data

Heater and cooler on opposite sides, to establish a symmetric gradient

Gradient setup superior to two-plate choice design

12 zones per side (specular), 40cm2 each

Temperature Δ proportionally divided into 12 (in the method paper 15°C-40°C = 2.27°C per zone)

4 thermocouples embedded in the thermal gradient ring

The exact temperature gradient measured in real time

CCD-camera (included in the standard package, with its dedicated support) and ANYmaze video-tracking software

Behavior recorded automatically during 60 minutes

The system includes a set of 4 dual (visible/I.R.) lights Ideal for videotracking: perfect view even in the dark



Commands on the heater/cooler front panel

Preset Temperature Read-out

On LCD display on the front panel of the thermal units


Via ANY maze specific protocol for TGR



Universal input 85-264 VAC, 50-60Hz, 40W max



Temperature Range

Heating Plate: from 20°C to 65°C

Heating/cooling Plate: from 4°C to 65°C

Precision 0.5°C
Temperature feedback By 4 thermocouples monitored in real time by ANYmaze
Light Intensity (I.R.) Set by potentiometer with graded scale
Light Intensity (visible) Set by potentiometer with graded scale



Internal diameter 45 cm
Outer diameter 57,5 cm
Corridor Width 6 cm

Wall Height

24 cm

Material IR transparent acrylic





Shipping Weight


Packing Dimensions

100x80x70(h)cm (wooden pallet)



Warranty 35550 is covered by a 12-months warranty + 12 months after product registration
UB-Care Additional UB-Care can be added for other 12 or 24 months

"Currently available behavioral assays to quantify normal cold sensitivity, cold hypersensitivity and cold hyperalgesia in mice have created conflicting results in the literature.

Some only capture a limited spectrum of thermal experiences, others are prone to experimenter bias or are not sensitive enough to detect the contribution of ion channels to cold sensing because in mice smaller alterations in cold nociception do not manifest as frank behavioral changes.

To overcome current limitations, we have designed a novel device that is automated, provides a high degree of freedom, i.e. thermal choice, and eliminates experimenter bias. The device represents a thermal gradient assay designed as a circular running track. It allows discerning exploratory behavior from thermal selection behavior and provides increased accuracy by providing measured values in duplicate and by removing edge artifacts.

The assay delivers discrete information on a large range of parameters extracted from the occupancy of thermally defined zones such as preference temperature and skew of the distribution.

We demonstrate that the assay allows increasingly accurate phenotyping of thermal sensitivity in transgenic mice by disclosing yet unrecognized details on the phenotypes of TRPM8-, TRPA1- and TRPM8/A1-deficient mice." From "Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay (Touska et al. 2016)"

Other scientists, like the M. Tominaga team in Japan, the R. Russo in Naples and the Tegeder team in Germany showed applications in Parkinson’s disease and chronic pain, TRP knock-out mice behaviors and testing of molecule candidates to interact with TRP receptors, which have been shown to be a valuable pharmacological option several therapeutic areas.

35550 Thermal Gradient Ring


Runway and Thermal Device Assembly

35550-035 Visibile and IR Light
35550-050 Light and Camera Support
35550-325 Camera
E-AU 101 USB Key including Instruction Manual



Dedicated Video-tracking Software

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